Conversion between bromcresol green‐ and bromcresol purple‐measured albumin in renal disease
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چکیده
منابع مشابه
Improved automated method for determining serum albumin with bromcresol green.
An automated method for determining serum albumin with bromcresol green according to Doumas et al. [C/in. Chim. Acta 31, 87 (1971)] was simplified and improved for use in routine analysis. The manifold is simpler, there is better sample separation and less sample interaction, both within-run and day-to-day run precision are excellent, and results agree well with those obtained by electrophoresi...
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References 1. Bodansky 0, Schwartz MK. Phosphohexoseisomerase: clinical aspects. Methods Enzymol 1966;9:568-75. 2. Hilf R, Feldstein ML, Gibson SL, Savlov ED. A logistic model based on enzyme activities for the prediction of response of breast cancer patients to chemotherapy. Cancer 1982;50:1734-8. 3. Mitchell I, Deshpande N, Millis R, Rubens RD. The activity of phosphohexose isomerase in prima...
متن کاملComparison of bromcresol green and agarose protein electrophoresis for quantitation of serum albumin in multiple myeloma.
BACKGROUND The International Staging System for multiple myeloma has increased the importance of accurate measurement of serum albumin. Two common albumin assays, bromcresol green (BCG) and agarose gel protein electrophoresis (PEL), frequently yield discordant results, creating confusion regarding which assay is superior for use in myeloma. METHODS We measured albumin by BCG on a Roche Modula...
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We describe an adaptation of automated multiple flow-injection analysis instrumentation to an analysis for albumin in serum. The bromcresol green reaction was used to test the utility of the system. The approach yielded albumin results with excellent sensitivity, no measurable carryover, a relative standard deviation of less than 1%, good correlations with published procedures, and no measurabl...
متن کاملImproved specificity of serum albumin determination and estimation of "acute phase reactants" by use of the bromcresol green reaction.
The reaction of serum samples with bromcresol green proceeds in two steps. Albumin is responsible for the faster (less than 1 min) reaction; the slower (30-min) reaction is a measure of "acute phase reactant(s)" in serum. Serum is simply mixed with bromcresol green reagent and the absorbance is measured twice, immediately and at 60 min. Albumin concentrations, determined from the absorbance at ...
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ژورنال
عنوان ژورنال: Nephrology Dialysis Transplantation
سال: 2001
ISSN: 1460-2385,0931-0509
DOI: 10.1093/ndt/16.9.1925